Material, Stain, Result - Immunofluorescence

biopsy, distant normal skin

leukocytoclastic vasculitis

biopsy, lesional recommended

EM, lichen planus

biopsy, perilesional recommended

bullous lupus erythematosus, bullous pemphigoid, cicatricial pemphigoid, dermatitis herpetiformis, EBA, herpes gestationis, linear IgA dermatosis, pemphigus, PCT

bullous pemphigoid

IgG, complement in homogenous linear pattern at dermoepidermal junction

cicatricial pemphigoid

1.  IgG, complement in homogenous linear pattern at dermoepidermal junction

2.  multiple Igs, complement, and/or fibrin in homogenous linear pattern at dermoepidermal junction

dermatitis herpetiformis

granular, focal IgA at dermoepidermal junction

EB simplex

anti BP ag: dermal base     anti laminin: dermal base     anti type IV collagen, anti-LDA-1: dermal base

EB, dystrophic

anti BP ag: epidermal roof    anti laminin: epidermal roof      anti type IV collagen, anti-LDA-1: epidermal roof

EB, junctional

anti BP ag: epidermal roof      anti laminin: dermal base    anti type IV collagen, anti-LDA-1: dermal base

EBA

1.  IgG, complement in homogenous linear pattern at dermoepidermal junction

2.  multiple Igs, complement, and/or fibrin in homogenous linear pattern at dermoepidermal junction

erythema multiforme

intravascular IgM, complement, &/or fibrin in granular pattern

herpes gestationis

IgG, complement in homogenous linear pattern at dermoepidermal junction

IgA

DH, linear IgA dermatosis, chronic bullous dermatosis of childhood

IgA dermatosis

IgA at epidermal cell surface

IgG

pemphigus, BP, HG, CP, EBA, LE, LP, PCT

lichen planus

multiple Igs , complement, &/or fibrin in cytoid bodies in upper dermis

lupus erythematosus, bullous

multiple Igs, complement, and/or fibrin in homogenous linear pattern at dermoepidermal junction

pemphigus

IgG, complement at epidermal cell surface

porphyria cutanea tarda

multiple Igs , complement, &/or fibrin in interstitial peivascular &/or dermoepidermal junction

split skin findings

dermis only: EBA, bullous SLE, cicatricial pemphigoid (some)

epidermis & dermis: BP (rare)

epidermis only: BP, cicatricial pemphigoid (some), chronic bullous dermatosis of childhood

vasculitis

intravascular IgM, complement, &/or fibrin in granular pattern