Material, Stain, Result - Immunohistochemistry

k & l chains

bone-marrow derived

AACT

bone-marrow derived

adnexal carcinoma

sweat gland keratinocyte; keratin, CEA, EMA

AE1/AE3

cytokeratin; malignant peripheral nerve sheath tumor

ALL

CD10

B-cell marker

CD10, CD19, CD20, CD22, CD45RA, light chains

bcl-2

melanocyte

Ber-H2

large cell lymphoma

bone marrow derived

CD68, CD45, CD30, CD20, CD22, CD68, k & l chains, CD1-5, 7, 8, HAM56, MAC 387, lysozyme, AACT

CALLA

see CD10

CAM 5.2

Merkel cell

carcinoma

cytokeratin +

CD1

bone-marrow derived

CD1a (T6)

thymocyte and Langerhan’s cells

CD2

pan T-cell antigen

CD3

pan T-cell antigen

CD4

bone-marrow derived; T cell & macrophages, T helper cells

CD5

T-cell antigen, bone-marrow derived; B-cell subsets

CD7

bone-marrow derived; precursor T cells, T cell subsets, natural killer cells

CD8

bone-marrow derived; T cell subsets, natural killer cells, T suppresser cells

CD10 (CALLA)

precursor B cells, granulocytes; follicular cell lymphoma, ALL

CD19

pan B-cell antigen marker

CD20

pan B-cell antigen, bone-marrow derived

CD22

pan B-cell antigen, bone-marrow derived

CD25

activation & proliferation antigen

CD30 (Ki-1)

activation & proliferation antigen, bone-marrow derived; large cell lymphoma

CD34

DFSP

CD43 (Leu-22)

T cells, macrophages, granulocytes

CD45

bone-marrow derived

CD45RA

pan B-cell marker

CD68

bone-marrow derived

dermal cells

factor XIIIa, vimentin

DFSP

CD34 +, smooth muscle specific actin +/-, smooth muscle actin +/-

EMA

sweat gland keratinocyte, sebocyte

endothelium

CD34, factor VIII-related antigen, ulex europaeus, CD31

epithelial membrane antigen

perineuroma

factor XIIIa

dermal cells; DFSP

fibrous histiocytoma

CD34 +/-, smooth muscle specific actin +/-, smooth muscle actin +/-

follicular center cell lymphoma

CD10

glomus tumor

smooth muscle actin, smooth muscle specific actin +/-

HAM 56

bone-marrow derived

HHF-35

smooth muscle specific actin

HLA-DR

activation & proliferation antigen

keratinocyte, epidermal

cytokeratin, AE-1

keratinocyte, sweat gland

keratin, CEA, EMA

Ki-1

see CD30; large cell lymphoma

Ki-1/CD 30 +

anaplastic large cell lymphoma, Reed-Sternberg cells of Hodgkin’s d., lymphomatoid papulosis, angioimmunoblastic lymphadenopathy, peripheral T-cell lymphoma, large cell lymphoma, PLEVA, regressing atypical histiocytosis, lymphocytes exposed to phytohemagglutinin, EBV, human T-cell leukemia virus, S. aureus

Ki-67

activation & proliferation antigen

Langerhan’s cells

S100, Mab 010, CD1a (OKT-6) monoclonal Ab stain, Birbeck granules (electron micrograph), S-100 Ab

Langerhans cell histiocytosis

Langerhans cell; S100, Mab 010

L26

evaluate & confirm B cell presence

large cell lymphoma

Ber-H2

leiomyosarcoma

muscle; actin, desmin

Leu-22

see CD43

light chains

monotypic plasma cells

lymphoma

LCA +

lymphoma panels

fixed tissue: suspected T-cell lymphoma; UCHL-1, CD30, L26, light chains

frozen tissue: suspected T-cell lymphoma; CD1a, CD2, CD4, CD7, CD8, CD30, CD43

                        suspected B-cell lymphoma; CD2, CD20, CD22, kappa, lambda

lysozyme

bone-marrow derived

MAC 387

bone-marrow derived

malignant peripheral nerve sheath tumor

cytokeratin +/-, smooth muscle specific actin +/-, smooth muscle actin +/-, desmin +/-

mast cells

toluidine blue, Giemsa, conjugated avidin

melanocyte

S100 protein, HMB45, cathepsin D, bcl-2

melanoma

melanocyte; S100, HMB45, cathepsin D, bcl-2

Merkel cell

neuron-specific enolase, CAM 5.2, neurosecretory markers

Merkel cell carcinoma

Merkel cell; neuron-specific enolase, CAM 5.2, neurosecretory markers

metastatic cells

thyroglobulin, prostatic acid phosphatase, serotonin

muscle

actin, desmin

myoepithelial cell, myofibroblasts

muscle specific actin, vimentin, glial fibrillary acidic protein

nerve

S100 protein, Leu 7, myelin basic protein

neurofibroma

S100 +/-

neuron-specific enolase

Merkel cell; Merkel cell carcinoma, small cell carcinoma

perineuroma

epithelial membrane antigen

prostatic acid phosphatase

metastatic cells-prostatic carcinoma

rhabdomyosarcoma

muscle; actin, desmin

S-100

adipocytes, apocrine glands, carcinoid, chondrocytes, chondrosarcoma, chondroblastoma, clear cell sarcoma, choroma, eccrine glands (both myoepithelial cells & luminal cells), epithelial sarcoma, folliculostellate cells of adenophypophysis, glial cells, gliomas, Langerhan’s cells, liposarcoma, leiomyosarcoma, melanocytes, metastatic breast carcinoma, myxomatous neurothekioma, nerve, nevus cells, osteosarcoma, Schwann cells, synovial sarcoma (some spindle cells)

SCC, poorly differentiated

cytokeratin, AE-1

sebaceous neoplasms

sebocyte; lipase, EMA

sebocyte

lipase, EMZ

serotonin

metastatic cells-carcinoid

schwannoma

S100

small cell carcinoma

neuron-specific enolase, CAM 5.2, neurosecretory markers

solitary fibrous tumor

CD34 +, smooth muscle specific actin +/-, smooth muscle actin +/-

T6

see CD1a

TdT

immature B & T cells

thyroglobulin

metastatic cells-thyroid carcinoma

UCHL-1

pan T-cell marker; evaluate & confirm T-cell presence, best marker in paraffin sections

vimentin

dermal cells; DFSP